Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
Published in Nature Communications, 2021
Recommended citation: Bouvette, J.*, Liu, H.*, Du, X., Zhou, Y., Sikkema, A.P., Mello, J.F.R., Klemm, B.P., Huang, R., Schaaper, R.M., Borgnia, M.J. & Bartesaghi, A. (2021). "Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography." Nat. Commun., 12(1957). https://doi.org/10.1038/s41467-021-22251-8
Tomographic reconstructions of cryopreserved specimens enable in-situ structural studies. Here, the authors present the beam image-shift electron cryo-tomography (BISECT) approach that accelerates data collection speed and improves the map resolution compared to earlier approaches and present the in vitro structure of a 300 kDa protein complex that was solved at 3.6 Å resolution as a test case.
Recommended citation: Bouvette, J.*, Liu, H.*, Du, X., Zhou, Y., Sikkema, A.P., Mello, J.F.R., Klemm, B.P., Huang, R., Schaaper, R.M., Borgnia, M.J. & Bartesaghi, A. (2021). “Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography.” Nat. Commun., 12, 1957.